Objective: We studied the influence of bile duct obstruction (BDO) after 28 days on bone metabolism status and their modification by resveratrol in male Wistar rats. Methods: The rats were divided into 3 groups: Sham group with laparotomy and vehiculum application, group BDO—bile duct obstruction and vehiculum application, and group RBDO—bile duct obstruction and resveratrol application (10 mg/kg dose of resveratrol orally once daily). The bone mineral density (BMD; g/cm2) and body composition were measured by dual energy X-ray absorptiometry. The physiccal strenght of femur was examined by controlled break biomechanical testing. The osteocalcin, procollagen type I N-terminal propeptide (PINP) and carboxy-terminal collagen crosslinks (CTX) were analysed by EIA in the bone tissue homogenate. Results: The total BMD was lower in group BDO (0.176±0.005; p=0.01) and R-BDO (0.181±0.004; p=0.052) vs Sham (0.209±0.003). BMD of femur diaphysis in BDO group decreased significantly (p=0.01) but not in R-BDO animals (p=0.052). Body fat (g, median) was lower in BDO (19.0) and R-BDO (26.0) than in Sham (35.0). The force needed for fracture of femurs (N) significantly decreased in BDO (154±6) and R-BDO (151±13) vs Sham group (208±7). The force needed for neck fracture decreased in BDO (105±3) and R-BDO (115±8) vs Sham (135±9). The osteocalcin decrease in group R-BDO (2.9±0.1) vs Sham (3.7±0.4). Higher PINP/CTX were in BDO (20±
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