Rice is an important staple food and a rich source of carbohydrates for 90% of South and Southeast Asians. Geneticdiversity of naturally adopted upland lines along with few germplasm collections in Karnataka were studied basedon 13 SSR loci on chromosome 1. A total of 24 individuals belonging to 3 differentially adopted groups [Upland(8), landraces (8) and cultivated varieties (8)] had a moderate to high level of genetic variability at groups level withnumber of alleles ranging from 5 to 23 (average 11.5) and PIC from 0.51 to 0.94 (average 0.79). The heterozygosityvaried from 0.39 to as high as 0.70. Over all genetic diversity of 0.81 was revealed indicating a high level of geneticvariation among these individuals. At the similarity coefficient of 7, all the lines were clearly grouped in 3 clusters.All cultivated types grouped in one cluster (cluster 1) except Rasi and CTH 1 which were included in cluster 2 andcluster 3 respectively. Cluster 1 contained 9 cultivated genotypes, where as cluster 2 contained 11 land races andcluster 3 had 4 upland lines. Out of 4 upland lines one line (AC-35341) intermixed, with C1 and three lines (PS-229,JBT-37/4 and AC-35310) with C3. The study of SSR markers on chromosome 1 facilitated the classification of theselines according to their adaptability. The information about the genetics of these populations will be for specificchromosomes and will be very useful for rice breeding programs, especially for gene mapping and ultimately formarker assisted selection programs.