Nine bacterial colonies were screened for the Cr(VI) removal efficiency and out of these three bacterial strains Pseudomonas putida, Pseudomonas aeruginosa and Bacillus sp. were isolated from soil and used to remove Cr(VI) from aqueous solution. The effect of time and concentrations on the removal rate of hexavalent chromium were studied using batch experiment. Maximum Cr (VI) removal was noted 75.0% by Bacillus sp. at 10mg/l, 69.70% by Pseudomonas aeruginosa at 40mg/l and 90.88% by Pseudomonas putida at 10mg/l of synthetic solution, during 96 hours. Among these three bacteria, the maximum Cr(VI) removal was reported by Pseudomonas putida on lower concentration. On the basis of highest removal rate, Pseudomonas putida was selected and used for further chromium removal from samples. It was found to be removed the highest Cr(VI) by 82.92%, from effluent and 74.41% from soil during 96 hours. The present study depicts that bacteria removes chromium efficiently and this could be used for industrial waste management and other environmental contaminants.