In order to study the effect of turmeric rhizome powder (TU) and enzyme in some blood parameters of laying hens, an in vivo study was conducted. In a 5 * 2 completely randomized block design with factorial arrangement, 5 levels of TU (0.0, 0.05, 0.10, 0.15, and 0.20 %) and 2 levels of enzyme (0.0, and 0.05%) with 4 blocks (replicate) , 480, 100-week old laying hens for 4 weeks fed wheat-soybean meal based diets. Some serum blood parameters of laying hens including hematocrit value, triglyceride, total cholesterol, HDL and LDL-cholesterol were recorded at 104 weeks of age. Increasing dietary levels of TU with or without a dietary enzyme significantly decreased serum triglyceride, total cholesterol and LDL-cholesterol (P< 0.05). TU without enzyme significantly increased HDL-cholesterol. It was concluded that dietary supplementation of TU improves some of good indices of serum blood components in laying hens and might be used as an ingredient in laying hen diets for manipulating egg composition.