ISOLATION, IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF WHITE ROT FUNGUS AND HETEROLOGOUS EXPRESSION OF GENE ENCODING LACCASE

The objective of the present study was to isolate wild white rot fungus and characterize the organism with respect to laccase gene. White rot fungus was isolated from decay wood sample and was subjected to Phylogenetic analysis by using basidiomycete-specific ITS primers for identification. mRNA of laccase enzyme was isolated, amplified by RT-PCR with specific forward and reverse primer that targeted the nucleotides present in right and left border of the mRNA. Amplified gene was tried to be transformed in to E.coli cells and the transformants were subjected for colony PCR, plate assay and decolorisation assay. All the results very clearly indicated the presence of laccase gene in the isolated white rot fungus and also the successful transformation of the gene in to E.coli cells.