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BMB Reports  2012 

Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells

Keywords: Atopic dermatitis , Costimulatory molecules , DNFB , Macrophages , NC/Nga mice

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We evaluated the expression of the costimulatory moleculesCD80 and CD83 and major histocompatibility (MHC) class IIinduced by 2,4-dinitrofluorobenzene (DNFB) in the RAW264.7 macrophage cell line. In contrast to the previously reportedeffect of DNFB on dendritic cells, CD86 expression didnot change. Furthermore, we observed that the CD83 expressionlevel transiently increased and then decreased.Induction of CD80 and MHC class II molecule expression anda decrease in CD83 expression by DNFB in vitro were alsoconfirmed in splenocytes of BALB/c and NC/Nga mice.However, DNFB did not influence CD83 expression in peritonealCD11b+ cells from BALB/c or NC/Nga mice. Detailedin vivo experiments and further studies on the possible contributionof CD11b+ cells to induce atopic dermatitis (AD)would be helpful to attain a better understanding of ADpathogenesis.


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