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BMB Reports  2012 

p13 from group II baculoviruses is a killing-associated gene

Keywords: Autographa californica nucleopolyhedrovirus , Ha-p13 , Heliothis armigera single nucleocapsid nucleopolyhedrovirus , Leucania separata multinuclear polyhedrosis virus , Ls-p13

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p13 gene was first described in Leucania separata multinuclearpolyhedrosis virus (Ls-p13) several years ago, but the functionof P13 protein has not been experimentally investigated todate. In this article, we indicated that the expression of p13from Heliothis armigera single nucleocapsid nucleopolyhedrovirus(Ha-p13) was regulated by both early and late promoter.Luciferase assay demonstrated that the activity of Ha-p13promoter with hr4 enhancer was more than 100 times inheterologous Sf9 cells than that in nature host Hz-AM1 cells.Both Ls-P13 and Ha-P13 are transmembrane proteins. Confocalmicroscopic analysis showed that both mainly located in thecytoplasm membrane at 48 h. Results of RNA interferenceindicated that Ha-p13 was a killing-associated gene for hostinsects H. armigera. The AcMNPV acquired the mentionedkilling activity and markedly accelerate the killing rate whenexpressing Ls-p13. In conclusion, p13 is a killing associatedgene in both homologous and heterologous nucleopolyhedrovirus.


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