A study was conducted in the Department of Environmental Science, Tamil Nadu Agricultural University, Coimbatore, to transform the normal compost into bioactive compost through the addition of various substrates, which has multiple benefits to the crop system. The key players in this transformation process were Azotobacter, Pseudomonas, Phosphobacteria, composted poultry litter, rock phosphate and diluted spent wash. This enrichment process has increased the nutritive value of compost. The highest nitrogen content (1.75%) and phosphorus content (1.16%) was observed in the treatment T5 (compost enriched with composted poultry litter, spent wash, microbial inoculants and rock phosphate). The beneficial microorganism viz., Azotobacter, Pseudomonas and Phosphobacteria population were higher in the treatment T5 where all the inputs (composted poultry litter, microbial consortium, rock phosphate and spent wash) were added to the compost. The plant growth promoters viz., IAA and GA content was more in the treatment applied with spent wash and microbial inoculum. Beneficial microorganisms, composted poultry litter, rock phosphate and diluted spent wash contributes maximum level of nutrients and growth promoters to the compost with small expenses.