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Protective Effect of Leaf Extract of Trichilia connaroides on Hypercholesterolemia Induced Oxidative Stress

Keywords: Trichilia connaroides , pro-oxidant state , antioxidant , lipid peroxidation , hypercholesterolemias

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Abstract:

The current study envisaged evaluation of potential antioxidant role of chloroform and methanol extracts of leaves of Trichilia connaroides in hypercholesterolemia induced oxidative stress. Male Albino Wistar rats were rendered hypercholesterolemic by feeding them with high fat diet. Hypercholesterolemic animals were then treated orally, each day with chloroform and methanol extract of leaves of Trichilia connaroides (CETC and METC) (100 mg kg-1 b.wt.) for a period of eight weeks. At the end of the eight week, biomarkers (in hepatic tissue) of oxidative stress viz., products of lipid peroxidation (MDA), catalase, superoxide dismutase (SOD) and reduced glutathione (GSH). Hypercholesterolemic rats had significantly elevated levels of products of lipid peroxidation, LPO (p<0.001), 0.2184±0.0071 nmol mg-1 of protein, compared to normal animals (0.1610±0.0083 nmol mg-1 of protein). Significantly lower levels of catalase (p<0.01) 2.054±0.2234 units mg-1, normal 2.726±0.1236 units mg-1), SOD (p<0.001, 3.373±0.1653 units mg-1 of protein, (normal 4.906±0.0780) and GSH (p<0.001, 8.498±0.4805 μmoles mg-1 of protein (normal 12.69±0.63910) were observed in such animals indicating the development of pro-oxidant status in these animals. CETC and METC extract treated animals recorded significantly reduced levels of LPO (p<0.001, 0.1725±0.0094, 0.1744±0.0032 respectively) and significantly elevated levels of SOD (p<0.001, 4.705±0.1632, 4.752±0.1220) and GSH (p<0.01, 11.71±0.4930, p<0.001, 12.92±0.5890), respectively. Levels of LPO and of endogenous antioxidant enzyme levels were restored closer to that of normal animals, signifying reversal of oxidative stress. CETC and METC protected the animals against hypercholesterolemia induced oxidative stress.

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