The crude methanolic extract of the bark of Manilkara zapota (Molina) Standley (Family: Cucurbitaceae), was evaluated for itspossible anti-inflammatory activity and determination of total flavonoids content growing in Bangladesh. The anti-inflammatory activity was studied using carrageenan and histamine-induced rat paw edema test at different doses (200 and 400 mg/kg body weight) of the methanol extract. At the dose of 400 mg/kg body weight, the extract showed a significant anti-inflammatory activity both in the carrageenan and histamine-induced oedema test models in rats showing 59.72% and 60.0% reduction in the paw volume comparable (P<0.01) to that produced by the standard drug indomethacin (62.50% and 65.16%) at 4h respectively. The percentage inhibition of the oedema paw volume by the 400 mg/kg body weight of the extract was also statistically significant (P<0.05; P<0.01)compared favorably with the indomethacin treated animals at 1, 2 and 3 h in both models. The total flavonoids content were calculated as quite high in methanolic extract (169.37 mg/g of quercetin equivalent). Acute toxicity test showed that the plant might be safe for pharmacological uses. Therefore, the obtained results tend to suggest the acute anti-inflammatory activity as well as total flavonoids content from the methanolic extract of the bark of Manilkara zapota and thus provide the scientific basis for the traditional uses of this plant part as a remedy for pain and inflammations.