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Cost-effective Approaches for in vitro mass Propagation of Rauwolfia serpentina Benth. Ex Kurz.

Keywords: Rauwolfia serpentina , support matrix , glass beads , liquid medium , tissue culture , mass propagation

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Abstract:

The present study describes a highly efficient and a very cost effective micropropagation protocol for exsitu conservation of an important medicinal plant, Rauwolfia serpentina using glass beads as support matrix in liquid culture medium and as a potential alternative to agar and market grade sugar as carbon source. Glass beads can replace the conventionally used gelling agent agar without compromising the quality of the in vitro regenerated shoots/plantlets of R. serpentina. This practice could be helpful in achieving more than 95% reduction in cost media for the production of single regenerated shoot, moreover, the glass beads can be reused for indefinite time after proper acid wash.

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