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Long term stability of paraoxonase-1 and high-density lipoprotein in human serum

DOI: 10.1186/1476-511x-11-53

Keywords: Paraoxonase, High-density lipoprotein, Serum, Stability, Storage

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Abstract:

In this study the stability of PON1 activity has been tested in human serum samples during storage up to 12?months at 3 commonly used temperatures, -20°C, -70°C and ?196°C. It was found that the stability of the PON1 activity is constant during 12?months of storage at ?70°C and ?196°C. Storage at ?20°C resulted in a small but statistically significant decrease after 6?months to about 94% of its original value. Nonetheless, the rank order between the samples at T?=?0 and 12?months remained the same. The same temperature dependence was found for the associated high-density lipoprotein.It can be concluded that ?70°C is the right temperature for storage to maintain the PON1 activity for at least one year. Storage at a lower temperature in liquid nitrogen (?196°C) is not necessary.

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