: Temperature of aquatic environment is important for ensuring the normal metabolism of aquatic animals. It also affects blood vascular system of aquatic organisms. The present paper deals with the influence of temperature on the hemoglobin content of freshwater fish, Channa punctatus. The freshwater fish, Channa punctatus, were collected from Godavari River, Nanded (Maharashtra). The fishes were exposed to different temperatures viz. 15°, 20°, 25°, 30°, 35° and 40° C. The total hemoglobin contents were observed at different temperature stress. The values were compared with the fish’s acclimated to ambient temperature. The haemoglobin content was found to be increased as temperature increases. The total hemoglobin content was found to be decreased as temperature decreases.