: The present study reports the effects of aqueous extract of seed of Holarrhena antidysenterica for the management of streptozotocin (STZ) induced diabetes in rat. For the management of the carbohydrate metabolism in diabetes by the said extract, the activities of carbohydrate metabolic enzymes like glucose-6-phosphatase, glucose-6-phosphate dehydrogenase and hexokinase in liver along with quantification of glycogen in liver and skeletal muscle were measured and noted a significant recovery in respect to diabetic control group. As hyperlipidemia is one of the disorders of diabetes so, we have also assessed the serum levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDLc), very low density lipoprotein cholesterol (VLDLc) and high density lipoprotein cholesterol (HDLc). The said aqueous extract also resulted a significant recovery in the levels of above mentioned biosensors of lipid profile when treated to experimentally induce diabetic rat. The extract has no toxic effect in general which has been focused here by the monitoring of GOT and GPT activities in liver and kidney. The results of this study enlighted that the aqueous extract of said plant part has both antidiabetic and antihyperlipidemic activities. Industrial relevance: Diet therapy along with insulin or oral hypoglycemic agent, an important way of treatment for the management of diabetes though the hypoglycemic agents used in this purpose have several demerits. Plants have been used for the treatment of different diseases from ancient time in the world. Herbal drugs are mostly less side effect than the chemical drugs. At present, lots of herbal formulations are available in the market as tablet form consisting of all raw materials. Our study will help to the herbal industries to form a new herbal drug for the management of diabetes with less side effects and minimum cost as the plants are available in the nature. The screening of the active principal (s) of the plant part would be a more potent target drug against diabetes which will help the herbal pharma industries for the generation of new and more effective drug in this purpose.