The use of hemicellulolytic enzymes has recently attracted considerable interest as a substitute for chlorine chemicals in pulp bleaching in view of the environmental concerns. Fungal biotechnology opens a potpourri of strains for identification and secondary metabolite extractions. The present work aims in extraction of xylanase from novel microorganism(s) for its commercial application as biobleaching agent in paper and pulp industries. Eight xylanolytic fungi were isolated for the enzyme production. The culture conditions for enzyme production were optimized. The optimal enzyme activity was assayed for the xylanse enzyme. The results of the work were discussed with related references.