All Title Author
Keywords Abstract

Publish in OALib Journal
ISSN: 2333-9721
APC: Only $99


Relative Articles

Convenient Detection of the Citrus Greening (Huanglongbing) Bacterium ‘Candidatus Liberibacter asiaticus’ by Direct PCR from the Midrib Extract

Predictive Sequence Analysis of the Candidatus Liberibacter asiaticus Proteome

Molecular characterization of a mosaic locus in the genome of 'Candidatus Liberibacter asiaticus'

Conservation of Gene Order and Content in the Circular Chromosomes of ‘Candidatus Liberibacter asiaticus’ and Other Rhizobiales

Validation of ‘Variable Number of Tandem Repeat’-Based Approach for Examination of ‘Candidatus Liberibacter asiaticus’ Diversity and Its Applications for the Analysis of the Pathogen Populations in the Areas of Recent Introduction

Transcriptome comparison and gene coexpression network analysis provide a systems view of citrus response to ‘Candidatus Liberibacter asiaticus’ infection

Transcriptional and Microscopic Analyses of Citrus Stem and Root Responses to Candidatus Liberibacter asiaticus Infection

Comparative phylogenomics and multi-gene cluster analyses of the Citrus Huanglongbing (HLB)-associated bacterium Candidatus Liberibacter

Characterization of copy numbers of 16S rDNA and 16S rRNA of Candidatus Liberibacter asiaticus and the implication in detection in planta using quantitative PCR

Deciphering the Bacterial Microbiome of Citrus Plants in Response to ‘Candidatus Liberibacter asiaticus’-Infection and Antibiotic Treatments


PCR detection of Candidatus Liberibacter asiaticus, the agent of Huanglongbin or greening disease in citrus

Keywords: PCR detection , greening bacterium , Candidatus Liberibacter asiaticus , citrus greening disease

Full-Text   Cite this paper   Add to My Lib


The causal agent of Huanglongbing (HLB) or citrus greening disease (CGD) was detected from total DNA isolated from midrib of leaves of citrus trees from Delhi, Jammu and Maharashtra showing symptoms of yellowing or yellow mottling by PCR amplification of ribosomal protein gene. The amplicon was cloned in pGEM-T easy vector and sequenced. The clone was 703 nucleotide long and showed 100 % nucleotide sequence identity with part of b operon gene of Asian species of greening bacterium i.e. Candidatus Liberibacter asiaticus but differed from African species of greening bacterium i.e. Candidatus Liberibacter africanus. Our studies indicated that b operon gene of Asian species of greening bacterium is highly conserved and can be used for quick detection of greening bacterium in citrus plant for phytosanitary and certification programme.


comments powered by Disqus