The hepatoprotective activity of the ethanol extract of the leaf of Ptrospermum acerifolium was investigated in rats for carbon tetrachloride induced hepatotoxicity. Hepatotoxicity was induced in male Wistar rats by intraperitoneal injection of carbon tetrachloride (0.1 ml/kg/d p.o. for 14 d). Ethanol extract of P. acerifolium leaves were administered to the experimental rats (25 mg/kg/d p.o. for 14d). The Hepatoprotective effect of these extracts was evaluated by liver function biochemical parameters (total bilirubin, serum protein, alanine aminotransaminase, asparatate aminotransaminase and alkaline phosphates activites) and histopathological studies of liver. In ethanol extract-treated animals, the toxicity effect of carbon tetrachloride was controlled significantly by restoration of the levels of serum bilirubin and enzymes as compared to the normal and standard drug silymarin-treated groups. Histology of liver sections of the animals treated with the extracts showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration which further evidence the hepatoprotective activity.