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Preservation of Washed Spermatozoa of Mehsana Buck at Refrigeration Temperature

Keywords: Buck , Diluents , Ejeculates , Semen Preservation , Spermatozoa

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Abstract:

The study was done to understand the preservation of Washed Spermatozoa of Mehsana Buck at Refrigeration temperature. The washed spermatozoa from 78 semen ejaculates, 26 each from 3 Mehsana bucks, were preserved at 4 ± 10C temperature in SM and TCFEY diluents up to 72 hours and the physical characteristics of spermatozoa were studied to assess the suitability of diluents. The individual sperm motility and live spermatozoa decreased significantly whereas abnormal spermatozoa increased significantly (P<0.05) at each 12 hrs interval of preservation in two diluents. The effect of diluents on spermatozoan characteristics was less pronounced in SM than TCFEY diluent. Among the interaction studies, stage of preservation affected the live spermatozoa and their morphology. The findings suggested superiority of SM diluent over TCFEY for preservation of Mehsana buck semen. [Vet. World 2012; 5(5.000): 294-296]

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