全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

Confirmation of Chlamydophila abortus in infected cell culture using Indirect Immunofluorescence technique

Keywords: Chlamydophila abortus , Indirect immunofluorescence , McCoy cell lines , Monoclonal antibodies

Full-Text   Cite this paper   Add to My Lib

Abstract:

Chlamydophila abortus (C. abortus) is an important abortifacient agent in bovines and ovines. Clinical diagnosis of the disease is often difficult. An early diagnosis can be achieved based on direct demonstration of the organism in clinical material and through the cultural recovery of the organism in embryonated chicken egg. For confirmatory diagnosis antigen detection methods or serological techniques can be adopted. The present study is aimed at the confirmatory diagnosis of C. abortus infection by indirect immunofluorescence technique following the isolation of the organism in cell culture. Specific apple green fluorescing inclusions of C. abortus in McCoy cell lines was detected from 72 h to 96 h post infection employing anti-chlamydial group specific monoclonal antibodies. Thus, a confirmatory diagnosis of the infection was possible with this study. [Vet. World 2011; 4(10.000): 473-474]

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133