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A secreted serine protease of Paracoccidioides brasiliensis and its interactions with fungal proteins

DOI: 10.1186/1471-2180-10-292

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Abstract:

A cDNA (Pbsp) encoding a secreted serine protease (PbSP), was isolated from a cDNA library constructed with RNAs of fungal yeast cells recovered from liver of infected mice. Recombinant PbSP was produced in Escherichia coli, and used to develop polyclonal antibodies that were able to detect a 66 kDa protein in the P. brasiliensis proteome. In vitro deglycosylation assays with endoglycosidase H demonstrated that PbSP is a N-glycosylated molecule. The Pbsp transcript and the protein were induced during nitrogen starvation. The Pbsp transcript was also induced in yeast cells infecting murine macrophages. Interactions of PbSP with P. brasiliensis proteins were evaluated by two-hybrid assay in the yeast Saccharomyces cerevisiae. PbSP interacts with a peptidyl prolyl cis-trans isomerase, calnexin, HSP70 and a cell wall protein PWP2.A secreted subtilisin induced during nitrogen starvation was characterized indicating the possible role of this protein in the nitrogen acquisition. PbSP interactions with other P. brasiliensis proteins were reported. Proteins interacting with PbSP are related to folding process, protein trafficking and cytoskeleton reorganization.Serine protease is a class of peptidases widely distributed in all domains of life that use a serine residue at the active site to cleave peptides [1]. Serine proteases are associated with virulence and nutrient cycling in many pathogens. In the human pathogen Trichophyton rubrum seven serine proteases genes were detected, two of them encoding products able to cleave keratin, suggesting the importance of these proteases in the invasion process in the human host [2]. Also, a secreted serine protease from Microsporum canis was described. A serine protease inhibitor, as well as a monoclonal antibody directed to the protein inhibited fungal adherence to reconstructed interfollicular feline epidermis [3]. In the entomophatogenic fungus Magnaporthe grisea, the SPM1 serine protease is positively regulated during nitrogen sta

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