The present study analyses the effects of high copper concentrations on its mobility in the soils from the wine-growing regions of the Northwestern Croatia. The aims of this study were to determine the copper distribution in vineyard soils by single extraction procedures, and to relate the total, mobile and mobilisable copper content to several chemical soil characteristics using multiple regression analysis. The total Cu concentrations were determined by an aqua regia procedure. Two other methods were used for evaluating soil available copper: DTPA extraction and calcium chloride extraction. The physical and chemical characteristics (texture, organic C, pH, CaCO3, total P, CEC) were determined as well. Both DTPA- and CaCl2-extractable copper were largely explained by the total copper contents, and they were also highly correlated to each other. It was found that the DTPA-extractable copper decreased with increasing cation exchange capacity, but concentrations of CaCl2-extractable copper mainly depend on pH.