All Title Author
Keywords Abstract

Publish in OALib Journal
ISSN: 2333-9721
APC: Only $99

ViewsDownloads

Relative Articles

Comparasion of iles-iles and cassava tubers as a Saccharomyces cerevisiae substrate fermentation for bioethanol production

Genome-wide screening of the genes required for tolerance to vanillin, which is a potential inhibitor of bioethanol fermentation, in Saccharomyces cerevisiae

Construction of Novel Saccharomyces cerevisiae Strains for Bioethanol Active Dry Yeast (ADY) Production

One-pot bioethanol production from cellulose by co-culture of Acremonium cellulolyticus and Saccharomyces cerevisiae

Bioethanol production from intermediate products of sugar beet processing with different types of Saccharomyces cerevisiae

The Time Variation of Saccharomyces Cerevisiae Inoculation in Simultaneous Saccharification and Fermentation of Cocoa (Theobroma Cacao L.) Pod for Bioethanol Production

Research progress and strategies on bioethanol production from lignocellulose by consolidated bioprocessing (CBP) using Saccharomyces cerevisiae
酿酒酵母纤维素乙醇统合加工 (CBP) 的策略及研究进展

The Production of Bioethanol from Cashew Apple Juice by Batch Fermentation Using Saccharomyces cerevisiae Y2084 and Vin13

Development and application of Saccharomyces cerevisiae cell-surface display for bioethanol production
酿酒酵母细胞表面展示技术在燃料乙醇生产中的应用及研究进展

DILUTED ACID PRETREATMENT OF PINUS RADIATA FOR BIOETHANOL PRODUCTION USING IMMOBILIZED SACCHAROMYCES CEREVISIAE IR2-9 IN A SIMULTANEOUS SACCHARIFICATION AND FERMENTATION PROCESS

More...
BMC Genomics  2012 

Genome sequencing and genetic breeding of a bioethanol Saccharomyces cerevisiae strain YJS329

DOI: 10.1186/1471-2164-13-479

Keywords: Bioethanol , Saccharomyces cerevisiae , Stress , Genome , RNA-Seq

Full-Text   Cite this paper   Add to My Lib

Abstract:

Background Environmental stresses and inhibitors encountered by Saccharomyces cerevisiae strains are the main limiting factors in bioethanol fermentation. Strains with different genetic backgrounds usually show diverse stress tolerance responses. An understanding of the mechanisms underlying these phenotypic diversities within S. cerevisiae populations could guide the construction of strains with desired traits. Results We explored the genetic characteristics of the bioethanol S. cerevisiae strain YJS329 and elucidated how genetic variations in its genome were correlated with specified traits compared to similar traits in the S288c-derived strain, BYZ1. Karyotypic electrophoresis combined with array-comparative genomic hybridization indicated that YJS329 was a diploid strain with a relatively constant genome as a result of the fewer Ty elements and lack of structural polymorphisms between homologous chromosomes that it contained. By comparing the sequence with the S288c genome, a total of 64,998 SNPs, 7,093 indels and 11 unique genes were identified in the genome of YJS329-derived haploid strain YJSH1 through whole-genome sequencing. Transcription comparison using RNA-Seq identified which of the differentially expressed genes were the main contributors to the phenotypic differences between YJS329 and BYZ1. By combining the results obtained from the genome sequences and the transcriptions, we predicted how the SNPs, indels and chromosomal copy number variations may affect the mRNA expression profiles and phenotypes of the yeast strains. Furthermore, some genetic breeding strategies to improve the adaptabilities of YJS329 were designed and experimentally verified. Conclusions Through comparative functional genomic analysis, we have provided some insights into the mechanisms underlying the specific traits of the bioenthanol strain YJS329. The work reported here has not only enriched the available genetic resources of yeast but has also indicated how functional genomic studies can be used to improve genetic breeding in yeast.

Full-Text

comments powered by Disqus

Contact Us

service@oalib.com

QQ:3279437679

微信:OALib Journal