With increasing production of bio-gas and availability of anaerobically digested cattle feedlot manure (ADM), we need a better understanding of the impact of its application on crop production and the environment. The objective of this study was to investigate nutrient and heavy metal uptake by barley forage from soil amended with ADM. A four-year field study was conducted in southern Alberta to compare annual application of ADM liquid (ADML), and the solid fraction separated from the ADM (ADMS) to raw undigested cattle feedlot manure (CFMR). An unamended control (CK) was also included for comparison. Treatments were replicated four times using a split plot experimental design. All amendments were applied in spring each year prior to seeding and barley was grown and harvested at the soft dough stage for making cattle silage feed. All amendments were applied at rates supplying 100 or 200 kg N ha-1 yr-1, assuming 100% mineral N and 50% organic N was available to crops in the year of application. Averaged over four years, the highest yields were found from ADML (9.55 and 9.6 Mg ha-1 yr-1), and lowest from CK (6.93 Mg ha-1 yr-1), with ADMS and CFMR (7.80 to 8.66 Mg ha-1) in between. Contents and total uptake of nutrients and heavy metals (N, P, Cu and Zn) by barley forage from ADML were higher than ADMS and CFMR. and higher at 200 kg N ha-1 yr-1 than 100 kg N ha-1yr-1. Our data suggest that anaerobic digestion increases nutrients and heavy metal availability in cattle feedlot manure, but most increases occurred in the liquid fraction. Our data also suggest that the impact of organic amendment application on forage barley production is not only affected by the types of amendment used, but also by agronomic practices (e.g., seeding date) as well as growing conditions.