Ascities is the accumulation of free fluid in the peritoneal cavity. The chronic liver disease with portal hypertension, congestive cardiac failure tuberculosis and malignancy are important causes of ascities. Ascities fluid and serum were collected from malignant and non malignant patients biochemical parameters such as cholesterol, total protein and albumin radient were studied throughly. The results revealed that the level of cholesterol in malignant Ascities is silightly greater increase than non malignant. The total protien in both serum and ascitic fluid were slightly higher in malignant patients than non malignant person. Albumin gradient showed promises in Ascities differentiation. Further Ascities fluid samples were cultured in Nutrient Agar medium (NA) and bacterial populations were isolated and identified. The predominant species of bacteria were Escherichia coli, Steptrococcus aureus and Enterococcus fecalis from patients with spontaneous bacterial peritonitis. The present study inferred that there was a light fluctuation noticed in Ascities fluids biochemical parameter and microbial population.