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HPTLC Fingerprint Profile and Isolation of Marker Compound of Ruellia tuberosa

DOI: 10.1155/2012/180103

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Abstract:

The present study was aimed to identification, isolation, and quantification of marker in R. tuberosa (Acanthaceae). HPTLC fingerprinting was carried out for various extract of root, stem, and leaf of R. tuberosa. From the HPTLC fingerprint the florescent band (under 366?nm) at : 0.56 (mobile phase chloroform?:?toluene?:?ethyl acetate (6?:?3?:?1, v/v)) was found in leaf, root, and stem of R. tuberosa. So, the florescent band (under 366?nm) at : 0.56 was isolated as marker compound RT-F2 from root of R. tuberosa. The marker compound RT-F2 was quantified by using HPTLC technique. The percentage (W/W) amount of RT-F2 was found to 40.0% and 44.6% in petroleum ether and ethyl acetate extract of R. tuberosa roots, respectively. Further study is suggested to characterization and biological nature of marker compound. 1. Introduction Marker compound means chemical constituents within a medicinal that can be used to verify its potency or identity. For sometimes, the marker compounds may be described as active ingredients or chemicals that confirm the correct botanical identity of the starting material. It is very difficult to identify correct marker compounds for all traditional medicinals, because some medicinals have unknown active constituents and others have multiple active constituents. A chromatographic fingerprint of a herbal medicine is a chromatographic pattern of the extract of some common chemical components of pharmacologically active and/or chemical characteristics. By using chromatographic fingerprints, the authentication and identification of herbal medicines can be accurately conducted even if the amount and/or concentration of the chemically characteristic constituents is not exactly the same for different samples of drug. Hence it is very important to obtain reliable chromatographic fingerprints that represent pharmacologically active and chemically characteristic component of the herbal drug [1–5]. Ruellia tuberosa is an erect, suberect, or diffuse perennial herb up to 60–70?cm tall herb and belongs to family Acanthaceae, a native of Central America, introduced into Indian garden as ornament. It is used medicinally in West Indies, Central America, Guiana, and Peru. R. tuberosa is commonly known as “Cracker plant” [6–8]. In Siddha system of medicine, leaves are given with liquid copal as remedy for gonorrhea and ear diseases [9], used in stomach cancer [10]. Dried and ground roots in dose of two ounces cause abortion and also used in sore eyes [11]. The herb also exhibits emetic activity and employed substitute of ipecac, also used in bladder

References

[1]  http://www.bluepoppy.com/blog/blogs/blog1.php/what-are-marker-compounds.
[2]  P. S. Patil and R. Shettigar, “An advancement of analytical techniques in herbal research,” Journal of Advanced Scientific Research, vol. 1, no. 1, pp. 8–14, 2010.
[3]  Y. Z. Liang, P. Xie, and K. Chan, “Quality control of herbal medicines,” Journal of Chromatography B, vol. 812, no. 1-2, pp. 53–70, 2004.
[4]  E. S. Ong, “Chemical assay of glycyrrhizin in medicinal plants by pressurized liquid extraction (PLE) with capillary zone electrophoresis (CZE),” Journal of Separation Science, vol. 25, no. 13, pp. 825–831, 2002.
[5]  P. S. Xie, “A feasible strategy for applying chromatography fingerprint to assess quality of Chinese herbal medicine,” Traditional Chinese Drug Research & Clinical Pharmacology, vol. 12, no. 3, pp. 141–169, 2001.
[6]  C. N. Pandey, Medicinal Plants of Gujarat, Gujarat Ecological Education and Research Foundation, Gujarat, India, 2005.
[7]  Medicinal Plants of the Guiana's (Guyana, Surinam, French Guiana).
[8]  D. L. Chothani, M. B. Patel, H. U. Vaghasiya, and S. H. Mishira, “Review on Ruellia tuberosa (cracker plant),” Pharmacognosy Journal, vol. 2, no. 12, pp. 506–512, 2010.
[9]  L. Suseela and S. Prema., “Pharmacognostic study on Ruellia tuberosa,” Journal of Medicinal and Aromatic Plant Sciences, vol. 29, pp. 117–122, 2007.
[10]  M. B. Reddy, K. R. Reddy, and M. N. Reddy, “Ethnobotany of Cuddapah district, Andhra Pradesh, India,” International Journal of Pharmacognosy, vol. 29, no. 4, pp. 273–280, 1991.
[11]  B. D. Kirtikar and B. D. Basu, Indian Medicinal Plants, vol. 3, International Book Distributors, Deheradun, India, 1935.
[12]  The Wealth Of India, A Dictionary Of Indian, Raw Material and Industrial Product, Publication and Information Directorate, Council of Scientific and Industrial Research, New Delhi, India, 1972.
[13]  N. Y. Chiu and K. H. Chang, “The illustrated medicinal plants of Taiwan,” Mingtong Medical Journal, vol. 226, no. 1, 1995.
[14]  F. A. Chen, A. B. Wu, P. Shieh, D. H. Kuo, and C. Y. Hsieh, “Evaluation of the antioxidant activity of Ruellia tuberosa,” Food Chemistry, vol. 94, no. 1, pp. 14–18, 2006.
[15]  C. A. Lans, Creole remedies. Case studies of ethnoveterinary medicine in Trinidad and Tobago, Ph.D.Dissertation, Wageningen University, Wageningen, The Netherlands, 2001, no. 2992.
[16]  C. A. Lans, “Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus,” Journal of Ethnobiology and Ethnomedicine, vol. 2, article 45, pp. 1–11, 2006.
[17]  M. J. Balick, F. Kronenberg, A. L. Ososki et al., “Medicinal plants used by latino healers for women's health conditions in New York City,” Economic Botany, vol. 54, no. 3, pp. 344–357, 2000.
[18]  F. A. Chen, A. B. Wu, P. Shieh, D. H. Kuo, and C. Y. Hsieh, “Evaluation of the antioxidant activity of Ruellia tuberosa,” Food Chemistry, vol. 94, no. 1, pp. 14–18, 2006.
[19]  C. Wiart, M. Hannah, M. Yassim, H. Hamimah, and M. Sulaiman, “Anti-microbial activity of Ruellia tuberosa L,” American Journal of Chinese Medicine, vol. 33, no. 4, pp. 683–685, 2005.
[20]  S. Arun, P. Giridharan, A. Suthar, et al., Isolation of Tylocrebrine from Ruellia tuberosa through Bioassay Directed Column Chromatography and Elucidating its Anti-Cancer and Anti-Inflammatory Potential, 7th Joint Meeting of GA, AFERP, ASP, PSI & SIF, Athens, Greece, 2008.
[21]  L. S. R. Arambewela, R. Thambugala, and W. D. Ratnasooriya, “Gastroprotective activity of Ruellia tuberosa root extract in rats,” Journal of Tropical Medicinal Plants, vol. 4, no. 2, pp. 191–194, 2003.
[22]  M. A. Alam, N. Subhan, M. A. Awal et al., “Antinociceptive and anti-inflammatory properties of Ruellia tuberosa,” Pharmaceutical Biology, vol. 47, no. 3, pp. 209–214, 2009.
[23]  C. F. Lin, Y. L. Huang, L. Y. Cheng, et al., “Bioactive flavonoid from Ruellia tuberosa,” The Journal of Chinese Medicine, vol. 17, no. 3, pp. 103–109, 2006.
[24]  S. S. Subramanian and A. G. R. Nair, “Apigenin glycoside from thunbergia fragrans and Ruellia tuberosa,” Current Science, p. 480, 1974.
[25]  R. S. Singh, H. S. Pandey, B. K. Singh, and R. P. Pandey, “A new triterpenoid from Ruellia tuberosa linn,” Indian Journal of Chemistry B, vol. 41, no. 8, pp. 1754–1756, 2002.
[26]  C. K. Andhiwal and R. P. Chandra Haas Varshney, “Phytochemical investigation of Ruellia tuberosa L,” Indian Drugs, vol. 23, no. 49, 1985.
[27]  C. K. Kokate, Practical Pharmacognosy, Vallabh Prakashan, New Delhi, India, 4th edition, 2005.
[28]  H. Wagner, S. Blade, and G. M. Zgainsky, Plant Drug Analysis, Springer, Great Britain, UK, 1984.
[29]  E. Stahl, “Thin layer chromatography,” in A Laboratory Hand Book, Springer, Berlin, Germany, 2nd edition, 1965.

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