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Identification of a novel Rev-interacting cellular protein

DOI: 10.1186/1471-2121-6-20

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Yeast-two hybrid screening of a T-cell cDNA library with Rev as bait led to isolation of a novel human cDNA product (16.4.1). 16.4.1-containing fusion proteins showed predominant cytoplasmic localization, which was dependent on CRM1-mediated export from the nucleus. Nuclear export activity of 16.4.1 was mapped to a 60 amino acid region and a novel transport signal identified. Interaction of 16.4.1 with Rev in human cells was shown in a mammalian two-hybrid assay and by colocalization of Rev and 16.4.1 in nucleoli, indicating that Rev can recruit 16.4.1 to the nucleus/nucleoli. Rev-dependent reporter expression was inhibited by overexpressing 16.4.1 and stimulated by siRNAs targeted to 16.4.1 sequences, demonstrating that 16.4.1 expression influences the transactivation function of Rev.These results suggest that 16.4.1 may act as a modulator of Rev activity. The experimental strategies outlined in this study are applicable to the identification and biological characterization of further novel Rev-interacting cellular factors.The human immunodeficiency virus (HIV) Rev protein is a small (116 amino acids) post-transcriptional activator of expression of incompletely spliced and unspliced HIV mRNAs. Since these HIV transcripts direct production of progeny virions, Rev is a crucial factor in HIV replication (for overview see [1]). Rev interacts with HIV mRNAs by binding to a structured RNA element called the RRE (Rev response element). Rev offsets the activities of inhibitory sequences (INS) in HIV-1 mRNAs [2,3] and promotes their export to the cytoplasm. Once in the cytoplasm, Rev may also stimulate production of viral proteins on translational level (reviewed in [4]).Rev characteristically localizes to the nucleus, where it accumulates in nucleoli. However, a proportion of the Rev molecules expressed in a cell continuously shuttles between nucleus and cytoplasm by using active transport mechanisms both for entry into and exit from the nucleus.Mutational analyses of the

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