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BMC Cancer  2010 

Alterations of the extracellular matrix in ovarian cancer studied by Second Harmonic Generation imaging microscopy

DOI: 10.1186/1471-2407-10-94

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Abstract:

For this investigation we use Second Harmonic Generation (SHG) imaging microcopy to study changes in the structure of the ovarian ECM in human normal and malignant ex vivo biopsies. This method directly visualizes the type I collagen in the ECM and provides quantitative metrics of the fibrillar assembly. To quantify these changes in collagen morphology we utilized an integrated approach combining 3D SHG imaging measurements and bulk optical parameter measurements in conjunction with Monte Carlo simulations of the experimental data to extract tissue structural properties.We find the SHG emission attributes (directionality and relative intensity) and bulk optical parameters, both of which are related to the tissue structure, are significantly different in the tumors in a manner that is consistent with the change in collagen assembly. The normal and malignant tissues have highly different collagen fiber assemblies, where collectively, our findings show that the malignant ovaries are characterized by lower cell density, denser collagen, as well as higher regularity at both the fibril and fiber levels. This further suggests that the assembly in cancer may be comprised of newly synthesized collagen as opposed to modification of existing collagen.Due to the large structural changes in tissue assembly and the SHG sensitivity to these collagen alterations, quantitative discrimination is achieved using small patient data sets. Ultimately these measurements may be developed as intrinsic biomarkers for use in clinical applications.In 2008, there were an estimated 21,650 new cases of ovarian cancer in the United States and 15,520 deaths (Cancer Facts and Figures 2008, American Cancer Society, Database). Little is currently known about markers of premalignancy, early malignancy or early pathways for malignancy that could be potentially manipulated for prevention or early detection of ovarian cancer. Additionally, it is recognized that malignant cells with a specific and identifyi

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