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Evaluation of the effect of inactivation by microwave and autoclave in homeopathic medicines

Keywords: homeopathy , seed dynamics , microwave , autoclave

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Background: The Brazilian Pharmacopoeia defines the sterilization process as a "method" intended to remove or destroy all forms of life, animal or plant, macroscopic or microscopic, saprophytic or not, present in the product concerned, without ensuring the complete inactivation of toxins or cellular enzymes. Microwaves are electromagnetic waves with frequencies ranging between 300MHz (300x106 Hz) and 300 GHz (300x109 Hz) and wavelengths from 1 m to 1 mm[1]. They are waves that lie within the region between TV waves and the infrared region within the spectrum of electromagnetic waves. According to the Technical Standards Textbook for Homeopathic Pharmacy, glass tubes may be reused after washed with running and purified water and inactivated by autoclaving at 120oC for 30 minutes or by a dry air buffer at 180oC for 30 minutes or at 140oC for 1 hour [2]. Aims: Current experiment evaluates the influence of ultra-diluted Sulphur with and without inactivation by autoclaving and microwaving for certain variables in the germination and growth of sorghum (Sorghum bicolor L. Moench - cv TX623B). Methodology: Ten milliliters of Sulphur in homeopathic dinamizations (proposed by Hering - DH) 9DH, 18DH and 30DH inactivated by microwave and by autoclave heat, and control with water, were added to petri dishes in which 20 sorghum seeds were distributed. The experiment was conducted in a growth chamber (BOD) at 25oC and during a 16-h photoperiod. Double-blind methodology to avoid researcher ¢a a ¢s possible interferences or trends, coupled to statistic treatment at the end of the experiment, was employed. Data underwent variance analysis and means were compared by Scott-Knott ¢a a ¢s test at 5% probability. Results: Homeopathy Sulphur changed the evaluated parameters of 9DH, 18 DH and 30 DH dinamizations when compared to control (water). Differences existed with regard to effects of the different microwave-treated (M9DH, M18DH, M30DH) and autoclaving-treated (A9DH, A18DH, A30DH) dinamizations. Sulphur had no significant difference in the aerial length (CPA) when compared with that of control. In the case of the same homeopathic treatments previously undergoing autoclaving, the three dinamizations had a similar response to CPA. The same occurred with drugs submitted to microwaves. With regard to root ¢a a ¢s growth (CSR), treatment with Sulphur inhibited growth in the dinamizations 18 DH and 30 DH when compared to control. This did not occur with 9 DH which had the same effect as that of water. In the case of groups treated with medicines 30 DH and 9DH undergoing micro

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