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Isolation of Enterobacter sakazakii from Powdered Foods Locally Consumed in Nigeria

Keywords: Food , Enterobacter sakazakii , antibiotic , antibiotic , infant milk

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Abstract:

The presence Enterobacter sakazakii was investigated from powdered food samples using enrichment procedure of Enterobacteriaceae Enrichment Broth (EEB), Violet Red Bile Glucose Agar (VRBGA) and Tryptic Soy Agar (TSA). A total of 140 food samples were tested. E. sakazakii was isolated from 20/70 powdered infant foods, 13/50 powdered milk and 5/20 milk-based products, making a total of 27.1% prevalence. The isolates were tested for their susceptibility to 15 antibiotics. The results showed that streptomycin was the most effective (94.7%), followed by ofloxacin (92.1%), levofloxacin (84.2%), ciprofloxacin (79.0%), pefloxacin (79.0%) and gentamycin (65.8%). The organism was resistant to rifampicin and amoxicillin.

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