The presence Enterobacter sakazakii was investigated from powdered food samples using enrichment procedure of Enterobacteriaceae Enrichment Broth (EEB), Violet Red Bile Glucose Agar (VRBGA) and Tryptic Soy Agar (TSA). A total of 140 food samples were tested. E. sakazakii was isolated from 20/70 powdered infant foods, 13/50 powdered milk and 5/20 milk-based products, making a total of 27.1% prevalence. The isolates were tested for their susceptibility to 15 antibiotics. The results showed that streptomycin was the most effective (94.7%), followed by ofloxacin (92.1%), levofloxacin (84.2%), ciprofloxacin (79.0%), pefloxacin (79.0%) and gentamycin (65.8%). The organism was resistant to rifampicin and amoxicillin.