Isolation of Enterobacter sakazakii from Powdered Foods Locally Consumed in Nigeria

The presence Enterobacter sakazakii was investigated from powdered food samples using enrichment procedure of Enterobacteriaceae Enrichment Broth (EEB), Violet Red Bile Glucose Agar (VRBGA) and Tryptic Soy Agar (TSA). A total of 140 food samples were tested. E. sakazakii was isolated from 20/70 powdered infant foods, 13/50 powdered milk and 5/20 milk-based products, making a total of 27.1% prevalence. The isolates were tested for their susceptibility to 15 antibiotics. The results showed that streptomycin was the most effective (94.7%), followed by ofloxacin (92.1%), levofloxacin (84.2%), ciprofloxacin (79.0%), pefloxacin (79.0%) and gentamycin (65.8%). The organism was resistant to rifampicin and amoxicillin.