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Collection and Grading of Bovine Cumulus-oocyte-complexes (COCs) from Slaughter House Ovaries in View of in vitro Maturation, Fertilization and Culture

Keywords: in vitro maturation in vitro fertilization , in vitro culture , ovum pick up

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The research was carried out at the Animal Breeding and Genetics Laboratory under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh. It was conducted with a view to establish some relationship between ovarian condition in terms of the presence or absence of corpus luteum and morphological quality of the COCs as well as the relationship between follicular diameter and quality of COCs. Significantly highest (p<0.01) number of follicles was found in type III ovaries. The number of follicles measuring 2-6 mm diameter was observed to be significantly higher (p<0.01) in type III than type II and type I ovaries. Moreover, grade A and grade B COCs were significantly highest (p<0.01) in number in 2-6 mm diameter follicles. It was established that cumulus cells surrounding the oocytes favour to a less or greater extent the IVM (In vitro maturation), IVF (In vitro fertilization) of oocytes and subsequent IVC (In vitro culture) of zygotes. On the basis of the study, it is concluded that type III ovaries having no corpus luteum may be suggested for obtaining good quality Cumulus-oocyte-complexes (COCs) in experiment for IVM, IVF and subsequent IVC.


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