Tannase production from Aspergillus oryzae NCIM 1032 using mixture of Jamun (Syzigium cumini) and Babul (Acacia nilotica) stem barks under solid state fermentation.

Tannase have been produced using Aspergillus oryzae NCIM 1032. Enzyme production was carried out under Solid State Fermentation using different tannin rich substrates like some tree barks i.e. Arjuna (Terminalia arjuna), Babul (Acacia nilotica), Jamun (Syzygium cumini), Mulberry, Chiku stem barks have been used. The mixture of Jamun and Babul bark in the ratio of 4:6 was found to be best substrates for the enzyme production under SSF. Metabolic heat generated due to fermentation was equally distributed throughout the substrate bed by agitation in rotating drum bioreactor and enzyme production increased at speed of 75 rpm and at intermittent agitation (2 min/day).The yield was increased by 16% with all these optimized parameters; it is about 116 U/g dry substrate. This is the first report on production of tannase by the combination of Jamun and Babul tree barks under agitated conditions.