Commercial poultry production is low in Kogi State even before the advent of highly pathogenic avian influenza (HPAI H5N1) outbreak in Nigeria. The low level of poultry production has persisted long after the socio-economic impacts of HPAI had improved. A study was conducted among 94 poultry stakeholders in the state with the use of questionnaire to assess their knowledge of poultry diseases, biosecurity and poultry husbandry practices in six Local Government Areas of Kogi State. The findings showed that 60.0% of poultry production was rural while the rest were backyard (semi commercial) poultry. About 64.7% of poultry kept were under extensive management with the commonest diseases seen under this management system being Newcastle disease (62.9%), Coccidiosis (52.3%), Fowl pox (46.9%), Gumboro disease (39.1%) and Fowl typhoid (36.1%). Biosecurity was poor as 92.9% of respondents did not have footbath or hand wash disinfection; 70% would throw away poultry litter in the refuse dump; 12% would use the poultry litter as manure while 11% would sell out the litter. In addition, 64.7% of the poultry farmers obtained their rearing stock from the live bird market and other unknown sources while only 35.3% obtained theirs from the hatchery. The findings of this study showed that the low level of commercial poultry production in Kogi State might be due to the impacts of diseases and poor husbandry practices undertaken by the farmers. It is recommended that government should train poultry farmers on biosecurity, disease prevention and the adoption of modern husbandry practices suitable for the traditional poultry production system.