The objective of this study is to analyze the influence of leadership styles on an organizational culture, teamwork and organizational performance. Behavior and how a head department plays a role of a leader will determine people work quality. There are findings that in universities, not all leaders are able to comprehensively understand what program department scope’s is. The sample was determined by proportional random sampling totaled 139 people. The variables of study include leadership styles, teamwork, organizational culture, and organizational performance. Data were collected by questionnaires, analysis using Structural Equation Modeling (SEM). The results showed leadership style variable does not directly influence organizational performance of program department, but indirectly through the influence of organizational culture and teamwork. Teamwork variables impact directly or indirectly through organizational culture to organizational performance of program department. Suggestions from this research are there is a need to develop the leadership culture through example, attitude and behavior in utilizing existing resources in the faculties, program departments and unit program.