Evaluation of the effect of three disinfectants on removing HBV contamination

Background and Aim: Infection control is an important issue in dentistry. Without an efficient infection control, pathogens left on instruments and working surfaces will have potential danger to patients’ health. In this research, antiviral effect of three disinfectants: 0.5% sodium hypochlorite 0.05% sodium hypochlorite and Deconex 50 AF, on HBV was investigated. Materials and Methods: In this interventional (before-after) study; serums of 26 HBV positive patients were analyzed by PCR HBV analysis and 9 contaminated species were obtained to test three disinfectants. 36 agar plates were prepared with the contaminated serums. 27 of the plates were disinfected in 3 separate groups with the above mentioned solutions. Nine remaining plates were not disinfected (control). Swabs wetted by BSAS (Bovine Serum Albumin Sodium Chloride) medium were applied on the surface of the plates and the were kept in the transferred medium and sent to virology-lab of Pasteur Institute. HBV DNA were detected by commercial kit of HBV PCR (polymerase chain reaction) method. Data were analyzed by Cochrane test with p<0.05 as the limit of significance. Results: None of samples disinfected with 0.5% sodium hypochlorite showed contamination. 11/1% of samples disinfected with 0.05% sodium hypochlorite and 44/4% of samples disinfected with Deconex 50 AF remained contaminated. Statistical analysis showed a significant difference between 0.5% sodium hypochlorite and the other groups. Conclusion: Our findings revealed that 0.5% sodium hypochlorite solution is a strong and efficient disinfectant against HBV. Key Words: HBV; Sodium hypochlorite