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Life cycle control of Chlamydomonas reinhardtii

DOI: 10.1186/gb-spotlight-20030423-01

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Abstract:

Huang and Beck generated a Phot RNA interference (RNAi) knockdown mutant with transformants expressing only 10% of wild-type levels of Phot. When assayed, the mutant had reduced levels of conversions of pre-gametes to gametes. Expression analysis of the late-stage gametogenesis genes revealed reduced mRNA levels in the RNAi algae, indicating that phototropin serves as the photoreceptor for the induction of these genes. Examination of the reactivation of dark-activated RNAi and wild-type gametes by illumination suggests that phototropin also functions in the restoration of gamete mating ability. Furthermnore, zygotes generated from wild-type and RNAi gametes were subjected to light-induced germination. The degree of zygote germination was distinctly lower when RNAi gametes were used, suggesting that Phot also mediates light-induced zygote germination. The authors propose that phototropin is the photoreceptor that controls the blue-light-dependent sexual differentiation of C. reinhardtii."Because the reactivation of dark-inactivated gametes does not require protein synthesis, it has been hypothesized that blue light activates proteins of the flagella involved in sexual agglutination by some chemical modifications. This finding opens up the opportunity to analyze the consequences of phototropin activation in flagella, i.e., a system of reduced complexity," conclude the authors.

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