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The sea urchin joins the genome era

DOI: 10.1186/gb-2000-1-6-reports0081

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The cornerstone of the sea urchin project was the construction of a contiguous set of bacterial artificial chromosomes (BACs) spanning the entire genome. To create a genomic scaffold, BAC clones were aligned using overlapping end sequences and additional sequence-tagged sites, then checked by comparing restriction enzyme digests. Analysis of the end sequences allowed the authors to estimate that the sea urchin genome contains about 27,000 genes, consistent with the urchin's genome being about one quarter the size of a human's. In addition to the BAC map, cDNA libraries were generated from several developmental stages and from particular organs. Each library was arrayed onto filters that are now available for hybridization screening. The genome project also includes a cDNA database that will expand as data from analyzed cDNAs become available.Further information about the Sea urchin genome project is available.The good news is that a complete BAC map of the genome makes the sea urchin ready for whole-genome sequencing. Unfortunately, it is not clear if such an effort will be undertaken any time soon. As the sea urchin genome is large - about a quarter the size of the three billion bases of the human genome - determining its entire sequence would require a significant effort. So far, only widely used model organisms such as the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster have received so much attention. The latest focus is on the human genome, with the mouse likely to be next. Where the sea urchin fits into these sequencing schemes is uncertain. Despite a few particular advantages as a model research organism, the urchin is now rarely used as such, and its popularity is unlikely to increase in the near future.One major feature of interest, however, is the sea urchin's phylogeny, as echinoderms are more closely related to chordates, and thus to the vertebrates, than are other invertebrate groups. Although only very distantly related, we an


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