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Critical Care  2002 

Assessment of tissue oxygen tension: comparison of dynamic fluorescence quenching and polarographic electrode technique

DOI: 10.1186/cc1457

Keywords: clinical measurement methodology, fiberoptic measurement, fluorescence quenching, ischemia, Stern–Volmer, tissue oxygenation

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Abstract:

Both systems measured PO2 accurately in the tonometer, and there was excellent correlation between them (r2 = 0.99). The polarographic system exhibited proportional bias that was not evident with the fluorescence method. In vivo, the fluorescence quenching technique provided a readily recordable signal that varied as expected.Measurement of tissue PO2 using fluorescence quenching is at least as accurate as measurement using the Eppendorf needle electrode in vitro, and may prove useful in vivo for assessment of tissue oxygenation.Accurate measurement of PO2 in biologic tissues has been of interest to both researchers and clinicians for many years [1]. For basic scientists measurement of PO2 provides insight into the complexities of oxygen flux at the tissue level, whereas for clinicians it moves the monitoring window a step closer to the cell. PO2 monitoring has been exploited most effectively by radiation oncologists, who have used intratumoral PO2 measurements to plan and guide radiotherapy [2]. Many articles in the anesthesia and critical care literature report the application of different technologies designed to measure tissue PO2[1,3,4,5,6,7,8,9,10,11,12,13,14], but the clinical use of PO2 measurement has largely been limited to assessment of brain tissue [15,16].Existing technologies for measuring tissue PO2 are either too expensive for everyday clinical use [14] or are based on polarographic principles [17], meaning that oxygen is consumed in the measurement process. In time this oxygen consumption affects the signal itself, and this effect persists as tissue PO2 decreases, perhaps making polarographic devices less suitable for detection of tissue hypoxia. We hypothesized that a PO2 measurement technique based on dynamic fluorescence quenching would provide a way to overcome the limitations of the current polarographic technique. We report here a head-to-head bench comparison of PO2 measurement using polarography versus measurement using dynamic fluorescence

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