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Protective effect of fermented papaya preparation on stress-induced acute gastric mucosal lesion

DOI: 10.4236/jbpc.2012.34038, PP. 311-316

Keywords: Acute Gastric Mucosal Lesion, Fermented Papaya Preparation, Oxidative Stress, Inflammation

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Abstract:

Stress ulceration is single or multiple mucosal defects with/without bleeding from the gastric mucosa during the physiologic stress. Oxidative stress (OS) is a key pathogenic factor in psychogenic stress-induced acute gastric mucosal lesion (AGML). Fermented papaya preparation (FPP) is reported to have oxygen radical scavenging activity and is effective in OS-related diseases. Here, we investigated the protective effects and the mechanism of action of FPP on stress-induced AGML in rats, induced by water immersion restraint stress (WIRS). Exposure of rats to 6-hour WIRS resulted in the appearance of splinter hemorrhages and mucosal lesions in the stomach. WIRS induced significant increase in lipid peroxidation and decrease in superoxide dismutase-like activity in both the plasma and gastric mucosa. WIRS also significantly increased myeloperoxidase activity together with Nuclear factor-kappaB (NF-kB) activation in gastric mucosa. FPP reduced all the above changes. The results suggest that oral administration of FPP provides protection against WIRS-induced acute gastric mucosal lesions through its anti-oxidative and anti-inflammatory properties.

References

[1]  Takagi, K. and Okabe, S. (1968) The effects of drugs on the production and recovery processes of the stress ulcer. The Japanese Journal of Pharmacology, 18, 9-18. doi:10.1254/jjp.18.9
[2]  Kwiecień, S., Brzozowski, T. and Konturek, S.J. (2002) Effects of reactive oxygen species action on gastric mucosa in various models of mucosal injury. Journal of Pysiology and Pharmacology, 52, 39-50.
[3]  Hamaguchi, M., Watanabe, T., Higuchi, K., Tominaga, K. Fujiwara, Y. and Arakawa, T. (2001) Mechanisms and roles of neutrophil infiltration in stress-induced gastric injury in rats. Digestive Diseases and Science, 46, 2708-2715. doi:10.1023/A:1012779530004
[4]  Nishida, K., Ohta, Y., Kobayashi, T. and Ishiguro, I. (1997) Involvement of the xanthine-xanthine oxidase system and neutrophils in the development of acute gastric mucosal lesions in rats with water immersion restraint stress. Digestion, 58, 340-351.
[5]  Ohta, Y. and Nishida, K. (2003) Protective effect of coad-ministered superoxide dismutase and catalase against stress-induced gastric mucosal lesions. Clinical and Experimental Pharmacology and Physiology, 30, 545-550. doi:10.1046/j.1440-1681.2003.03871.x
[6]  Hirota, M., Inoue, M., Ando, Y., Hirayama, K., Morino, Y., Sakamoto, K., Mori, K. and Akagi, M. (1989) Inhibition of stress-induced gastric injury in the rat by glutathione. Gastroenterology, 97, 853-859.
[7]  Jia, Y.T., Ma, B., Wei, W., Xu, Y., Wang, Y., Tang, H.T. and Xia, Z.F. (2007) Sustained activation of nuclear factor-kB by reactive oxygen species is involved in the pathogenesis of stress-induced gastric damage in rats. Critical Care Medicine, 35, 1582-1591. doi:10.1097/01.CCM.0000266824.82280.17
[8]  Noda, Y., Murakami, S., Mankura, M. and Mori, A. (2008) Inhibitory effect of fermented papaya preparation on hydroxyl radical generation from methylguanidine. Journal of Clinical Biochemistry and Nutrition, 43, 185-190. doi:10.3164/jcbn.2008062
[9]  Imao, K., Wang, H., Komatsu, M. and Hiramatsu, M. (1998) Free radical scavenging activity of fermented papaya preparation and its effect on lipid peroxide level and superoxide dismutase activity in iron-induced epileptic foci of rats. Biochemistry and Molecular Biology International, 45, 11-23.
[10]  Imao, K., Komatsu, M., Wang, H. and Hiramatsu, M. (1999) Inhibitory effect of fermented papaya preparation on oxidative DNA damage and tissue injury in the brain formed during iron-induced epileptogenesis in rats. Journal of Brain Science, 25, 71-77.
[11]  Zhang, J., Mori, A., Chen, Q. and Zhao, B.L. (2006) Fermented papaya preparation attenuates β-amyloid precursor protein: β-amyloid-mediated copper neurotoxicity in β-amyloid precursor protein and in β-amyloid precursor Swedish mutation overexpressing SH-SY5Y cells. Neuroscience, 143, 63-72. doi:10.1016/j.neuroscience.2006.07.023
[12]  Hiramoto, K., Imao, M., Sato, E., Inoue, M. and Mori, A. (2008) Effect of fermented papaya preparation on dermal and intestinal mucosal immunity and allergic inflammations. Journal of the Science of Food and Agriculture, 8, 1151-1157. doi:10.1002/jsfa.3190
[13]  Ohta,Y., Imai, Y., Kaida, S., Kamiya, Y., Kawanishi, M. and Hirata, I. (2010) Vitamin E protects against stress-induced gastric mucosal lesions in rats more effectively than vitamin C. Biofactors, 36, 60-69.
[14]  Abdallah, D.M., El-Abhar, H.S. and Abdel-Aziz, D.H. (2009) TEMPOL, a membrane-permeable radical scavenger, attenuates gastric mucosal damage induced by ischemia/ reperfusion: A key role for superoxide anion. European Journal of Pharmacology, 603, 93-97. doi:10.1016/j.ejphar.2008.11.057
[15]  Ohta, Y., Kobayashi, T., Nishida, K., Nagata, M. and Ishiguro, I. (1999) Therapeutic effect of Oren-gedoku-to extract on stress-induced acute gastric mucosal lesions in rats. Phytotherapy Research, 13, 588-592. doi:10.1002/(SICI)1099-1573(199911)13:7<588::AID-PTR505>3.0.CO;2-P
[16]  Iwasaki, Y., Matsui, T. and Arakawa, Y. (2004) The protective and hormonal effects of proanthocyanidin against gastric mucosal injury in wistar rats. Journal of Gastroenterology, 39, 831-837. doi:10.1007/s00535-004-1399-5
[17]  Bowie, A, O’Neill L.A. (2000) Oxidative stress and nuclear factor-κB activation: A reassessment of the evidence in the light of recent discoveries. Biochemical Pharmacology, 59, 13-23. doi:10.1016/S0006-2952(99)00296-8
[18]  Baldwin, A.S. Jr. (1996) The NF-kappa B and I kappa B proteins: New discoveries and insights. Annual Reviews Immunology, 14, 649-683.

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