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Isolation and Purification of a Novel Deca-Antifungal Peptide from Potato (Solanum tuberosum L. cv. Jopung) Against Candida albicans

DOI: 10.3390/ijms13044021

Keywords: AFP-J, partial acid digestion, Potide-J, antibiotic agent

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Abstract:

In a previous study, an antifungal protein, AFP-J, was purified from tubers of the potato ( Solanum tuberosum cv. L Jopung) and by gel filtration and HPLC. In this study, the functional peptide was characterized by partial acid digestion using HCl and HPLC. We obtained three peaks from the AFP-J, the first and third peaks were not active in the tested fungal strain. However, the second peak, which was named Potide-J, was active (MIC; 6.25 μg/mL) against Candida albicans. The amino acid sequences were analyzed by automated Edman degradation, and the amino acid sequence of Potide-J was determined to be Ala-Val-Cys-Glu-Asn-Asp-Leu-Asn-Cys-Cys. Mass spectrometry showed that its molecular mass was 1083.1 Da. Finally, we confirmed that a disulfide bond was present between Cys 3 and Cys 9 or Cys 10. Using this structure, Potide-J was synthesized via solid-phase methods. In these experiments, only the linear sequence was shown to display strong activity against Candida albicans. These results suggest that Potide-J may be an excellent candidate compound for the development of commercially applicable antibiotic agents.

References

[1]  Birk, Y. The Bowman-Birk inhibitor, trypsin- and chymotrypsin-inhibitor from soybeans. Int. J. Pept. Protein Res 1985, 25, 113–131.
[2]  Ye, X.Y.; Wang, H.X.; Ng, T.B. First chromatographic isolation of an antifungal thaumatin-like protein from French bean legumes and demonstration of its antifungal activity. Biochem. Biophys. Res. Commun 1999, 263, 130–134.
[3]  Benhamou, N.; Broglie, K.; Broglie, R.; Chet, I. Antifungal effect of bean endochitinase on Rhizoctonia solani: Ultrastructural changes and cytochemical aspects of chitin breakdown. Can. J. Microbiol 1993, 39, 318–328.
[4]  Vogelsang, R.; Barz, W. Purification, characterization and differential hormonal regulation of a β-1,3-glucanase and two chitinases from chickpea (Cicer arietinum L.). Planta 1993, 189, 60–69.
[5]  Kim, J.Y.; Park, S.C.; Kim, M.H.; Lim, H.T.; Park, Y.; Hahm, K.S. Antimicrobial activity studies on a trypsin chymotrypsin protease inhibitor obtained from potato. Biochem. Biophys. Res. Commun 2005, 330, 921–927.
[6]  Khalf, M.; Goulet, C.; Vorster, J.; Brunelle, F.; Anguenot, R.; Fliss, I.; Michaud, D. Tubers from potato lines expressing a tomato Kunitz protease inhibitor are substantially equivalent to parental and transgenic controls. Plant Biotechnol. J 2010, 8, 155–169.
[7]  Walker-Simmons, M.; Ryan, C.A. Immunological identification of proteinase inhibitors I and II in isolated tomato leaf vacuoles. Plant Physiol 1977, 60, 61–63.
[8]  Schirra, H.J.; Anderson, M.A.; Craik, D.J. Structural refinement of insecticidal plant proteinase inhibitors from Nicotiana alata. Protein Pept. Lett 2008, 15, 903–909.
[9]  Leah, R.; Tommerup, H.; Svendsen, I.; Mundy, J. Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J. Biol. Chem 1991, 246, 1564–1573.
[10]  Kamemura, K.; Furuichi, Y.; Umekawa, H.; Takahashi, H.C. Purification and characterization of novel lectins from Great Northern bean, Phaseolus ulgaris L. Biochim. Biophys. Acta 1993, 1158, 181–188.
[11]  Pouvreau, L.; Gruppen, H.; van Koningsveld, G.A.; van den Broek, L.A.M.; Voragen, A.G.J. The most abundant protease inhibitor in potato tuber (cv. Elkana) is a protease inhibitor from the Kunitz family. J. Agric. Food Chem 2003, 51, 5001–5005.
[12]  Valueva, T.A.; Revina, T.A.; Mosolov, V.V.; Mentele, R. Primary structure of potato Kunitz-type serine proteinase inhibitor. Biol. Chem 2000, 381, 1215–1221.
[13]  Sagaram, U.S.; Pandurangi, R.; Kaur, J.; Smith, T.J.; Shah, D.M. Structure-activity determinants in antifungal plant defensins MsDef1 and MtDef4 with different modes of action againstFusarium graminearum. PLoS One 2011, 6, doi:10.1371/journal.pone.0018550.
[14]  Bhattacharyya, A.; Mazumdar, S.; Leighton, S.M.; Babu, C.R. Kunitz proteinase inhibitor from Archidendron ellipticum seeds: Purification, characterization, and kinetic properties. Phytochemistry 2006, 67, 232–241.
[15]  Kim, J.Y.; Park, S.-C.; Hwang, I.; Cheong, H.; Nah, J.-W.; Hahm, K.-S.; Park, Y. Protease Inhibitors from Plants with Antimicrobial Activity. Int. J. Mol. Sci 2009, 10, 2860–2872.
[16]  Herzog, M.; Dorne, A.M.; Grellet, F. GASA, a gibberellin-regulated gene family from Arabidopsis thaliana related to the tomato GAST1 gene. Plant Mol. Biol 1995, 27, 743–752.
[17]  Park, C.J.; Park, C.B.; Hong, S.-S.; Lee, H.-S.; Lee, S.Y.; Kim, C. Characterization and cDNA cloning of two glycine- and histidine-rich antimicrobial peptides from the roots of shepherd’s purse, Capsella bursa-pastoris. Plant Mol. Biol 2000, 44, 187–197.
[18]  Tam, J.P.; Lu, Y.-A.; Yang, J.-L.; Chiu, K.-W. Anunusual structural motif antimicrobial peptides containing end-to-end macrocycle and cystein-knot disulfides. Proc. Natl. Acad. Sci. USA 1999, 96, 8913–8918.
[19]  Benchekroun, A.; Alami, M.; Ghadouan, M.; Lachkar, A.; Kasmaoui, H.; Marzouk, M.; Faik, M. Urinary candidiasis revealed by ureteral obstruction: Report of 2 cases. Ann. Urol (Paris) 2000, 34, 171–174.
[20]  Hoy, J.; Hsu, K.C.; Rolston, K.; Hopfer, R.L.; Luna, M.; Bodey, G.P. Trichosporon beigelii infection: A review. Rev. Infect. Dis 1986, 8, 959–967.
[21]  Moreno, M.; Segura, A.; García-Olmedo, F. Pseudothionin-St1, a potato peptide active against potato pathogens. Eur. J. Biochem 1994, 223, 135–139.
[22]  Laemmli, U.K.; Favre, M. Maturation of the head of bacteriophage T4. J. Mol. Biol 1973, 80, 575–579.
[23]  Lee, D.G.; Shin, S.Y.; Maeng, C.-Y.; Jin, Z.Z.; Kim, K.L.; Hahm, K.-S. Isolation and Characterization of a Novel Antifungal Peptide from Aspergillus niger. Biochem. Biophys. Res. Commun 1999, 263, 646–651.
[24]  Jahn, B.; Martin, E.; Stueben, A.; Bhakdi, S. Susceptibility testing of Candida albicans and Aspergillus species by a simple microtiter menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)- 2,5-diphenyl-2H-tetrazolium bromide assay. J. Clin. Microbiol 1995, 33, 661–667.
[25]  María, E.; Paloma, M.; María, Y.; Mireia, M.; Salvador, V.; Jose, F.M. Selectivity and antimicrobial action of bovine lactoferrin derived peptides against wine lactic acid bacteria. Food Microbiol 2009, 26, 340–346.
[26]  Moleyar, V.; Narasimham, P. Antifungal activity of some essential oil components. Food Microbiol 1986, 3, 331–336.
[27]  Pouvreau, L.; Gruppen, H.; Piersma, S.R.; van den Broek, L.A.M.; van Koningsveld, G.A.; Voragen, A.G.J. Relative abundance and inhibitory distribution of protease inhibitors in potato juice from cv. Elkana. J. Agric. Food Chem 2001, 49, 2864–2874.

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