The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. The colonization of P. gingivalis on JH8194-Ti was significantly lower than that on control- and blocking-Ti. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control- and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration.
Murakami, Y; Tamagawa, H; Shizukuishi, S; Tsunemitsu, A; Aimoto, S. Biological role of an arginine residue present in a histidine-rich peptide which inhibits hemagglutination of Porphyromonas gingivalis. FEMS Microbiol. Lett？1992, 77, 201–204. 1334017
Schliephake, H; Aref, A; Scharnweber, D; Bierbaum, S; Roessler, S; Sewing, A. Effect of immobilized bone morphogenic protein 2 coating of titanium implants on peri-implant bone formation. Clin. Oral. Impl. Res？2005, 16, 563–569, doi:10.1111/j.1600-0501.2005.01143.x.
Park, J; Suh, J; Chung, H. Effects of calcium ion incorporation on osteoblast gene expression in MC3T3-E1 cells cultured on microstructured titanium surfaces. J. Biomed. Mater. Res.: A？2008, 86, 117–126.
Becker, D; Geissler, U; Hempel, U; Bierbaum, S; Scharnweber, D; Worch, H; Wenzel, K. Proliferation and differentiation of rat calvarial osteoblasts on type I collagen-coated titanium alloy. J. Biomed. Mater. Res？2002, 59, 516–527, doi:10.1002/jbm.1265. 11774310
Degasne, I; Baslé, M; Demais, V; Huré, G; Lesourd, M; Grolleau, B; Mercier, L; Chappard, D. Effects of roughness, fibronectin and vitronectin on attachment, spreading, and proliferation of human osteoblast-like cells (Saos-2) on titanium surfaces. Calcif. Tissue. Int？1999, 64, 499–507, doi:10.1007/s002239900640. 10341022
Martin, H; Schulz, K; Bumgardner, J; Walters, K. XPS study on the use of 3-aminopropyltriethoxysilane to bond chitosan to a titanium surface. Langmuir？2007, 23, 6645–6651, doi:10.1021/la063284v. 17488131
Nikawa, H; Sadamori, S; Hamada, T; Okuda, K. Factors involved in the adherence of Candida albicans and Candida tropicalis to protein-adsorbed surfaces. An in vitro study using immobilized protein. Mycopathologia？1992, 118, 139–145, doi:10.1007/BF00437146. 1528229
Makihira, S; Yan, W; Ohno, S; Kawamoto, T; Fujimoto, K; Okimura, A; Yoshida, E; Noshiro, M; Hamada, T; Kato, Y. Enhancement of cell adhesion and spreading by a cartilage-specific noncollagenous protein, cartilage matrix protein (CMP/Matrilin-1), via integrin alpha1beta1. J. Biol. Chem？1999, 274, 11417–11423, doi:10.1074/jbc.274.16.11417. 10196235