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Insects  2012 

Transmission of a Gammabaculovirus within Cohorts of Balsam Fir Sawfly (Neodiprion abietis) Larvae

DOI: 10.3390/insects3040989

Keywords: Gammabaculovirus, Neodiprion abietis, nucleopolyhedrovirus, balsam fir sawfly, Abies balsamea, disease transmission, pest management

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Abstract:

Nucleopolyhedroviruses (NPV: Gammabaculovirus: Baculoviridae) of diprionid sawflies (Diprionidae: Hymenoptera) are highly host specific and only infect the midgut epithelium. While still alive, infected sawfly larvae excrete NPV-laden diarrhea that contaminates food sources. The diarrhea can then be consumed by conspecific larvae, resulting in rapid horizontal transmission of the virus. To better understand the efficacy of Gammabaculovirus-based biological control products, the horizontal spread of such a virus (NeabNPV) within cohorts of balsam fir sawfly ( Neodiprion abietis) larvae was studied by introducing NeabNPV-treated larvae into single-cohort groups at densities similar to those observed during the increasing (field study) and peak (laboratory study) phases of an outbreak. In field studies (~200 N. abietis larvae/m 2 of balsam fir ( Abies balsamea) foliage), NeabNPV-induced mortality increased positively in a density-dependent manner, from 23% (in control groups) to 51% with the addition of one first-instar NeabNPV-treated larva, to 84% with 10 first–instar-treated larvae. Mortality was 60% and 63% when one or 10 NeabNPV-treated third-instar larva(e), respectively, were introduced into groups. Slightly higher levels of NeabNPV-induced mortality occurring when NeabNPV-treated larvae were introduced into first- rather than third-instar cohorts suggests that early instars are more susceptible to the virus. In the laboratory (~1330 N. abietis larvae/ m 2 of foliage), NeabNPV-caused mortality increased from 20% in control groups to over 80% with the introduction of one, five or 10 NeabNPV-treated larvae into treatment groups of first-instar larvae.

References

[1]  Smith, D.R. Systematics, life history and distribution of sawflies. In Sawfly Life HistoryAdaptations to Woody Plants; Wagner, M.R., Raffa, K.F., Eds.; Academic Press: New York, NY, USA, 1993; pp. 3–32.
[2]  Wallace, D.R.; Cunningham, J.C. Diprionid sawflies. In Forest Insect Pests in Canada; Armstrong, J.A., Ives, W.G.H., Eds.; Natural Resources Canada: Ottawa, Canada, 1995; pp. 193–232.
[3]  Jehle, J.A.; Blissard, G.W.; Bonning, B.C.; Cory, J.S.; Herniou, E.A.; Rohrmann, G.F.; Theilmann, D.A.; Thiem, S.M.; Vlak, J.M. On the classification and nomenclature of baculoviruses: A proposal for revision. Arch. Virol. 2006, 151, 1257–1266, doi:10.1007/s00705-006-0763-6.
[4]  Knerer, G.; Atwood, C. Diprionid sawflies: Polymorphism and speciation. Science 1973, 179, 1090–1099.
[5]  Moreau, G. Past and present outbreaks of the balsam fir sawfly: An analytical review. Forest Ecol. Manag. 2006, 221, 215–219, doi:10.1016/j.foreco.2005.09.020.
[6]  Canadian Council of Forest Ministers. Forest Insect National Tables for Areas of Moderate to Severe Defoliation. Available online: http://nfdp.ccfm.org/insects/national_e.php (Accessed on 27 July 2012).
[7]  Smith, H.V.E. Annual Report of the Fire Patrol to the Annual Executive Meeting of The Newfoundland Forest Protection Association; Blackmore Printing Co. Ltd. Grand Falls: Newfoundland, Canada, 1947; pp. 46–63.
[8]  Carroll, W.J. Some aspects of the Neodiprion abietis complex in Newfoundland. Ph.D. dissertation, State University College of Forestry, Syracuse University, Syracuse, NY, USA, 1962.
[9]  Duffy, S.P.; Young, A.M.; Morin, B.; Lucarotti, C.J.; Koop, B.F.; Levin, D.B. Sequence analysis and organization of the Neodiprion abietis nucleopolyhedrovirus genome. J. Virol. 2006, 80, 6952–6963, doi:10.1128/JVI.00187-06.
[10]  Moreau, G.; Lucarotti, C.J.; Kettela, E.G.; Thurston, G.S.; Holmes, S.; Weaver, C.; Levin, D.B.; Morin, B. Aerial application of nucleopolyhedrovirus induces decline in increasing and peaking populations of Neodiprion abietis. Biol. Contr. 2005, 33, 65–73, doi:10.1016/j.biocontrol.2005.01.008.
[11]  Lucarotti, C.J.; Moreau, G.; Kettela, E.G. Abietiv?—A viral biopesticide for control of the balsam fir sawfly. In Biological Control: A Global Pespective; Vincent, C., Goettel, M., Lazarovits, G., Eds.; CABI International: Wallingford, UK, 2007; pp. 353–361.
[12]  Lucarotti, C.J.; Morin, B.; Graham, R.I.; Lapointe, R. Production, application, and field performance of Abietiv?, the balsam fir sawfly nucleopolyhedrovirus. Virol. Sinica. 2007, 22, 163–172, doi:10.1007/s12250-007-0018-z.
[13]  Moreau, G. The Influence of Forest Management on Defoliator Populations: A Case Study with Neodiprion Abietis in Pre-commercially thinned and Natural Forest Stands. Ph.D. dissertation, Faculty of Forestry and Environmental Management, University of New Brunswick, New Brunswick, Canada, 2004.
[14]  Federici, B.A. Baculovirus pathogenesis. In The Baculoviruses; Miller, K.L., Ed.; Plenum Publishing Corporation: New York, NY, USA, 1997; pp. 33–59.
[15]  Lucarotti, C.J.; Whittome-Waygood, B.H.; Lapointe, R.; Levin, D.B. Pathology of a Gammabaculovirus in its natural balsam fir sawfly (Neodiprion abietis) host. Psyche 2012. submitted.
[16]  Campbell, C.S.; Quiring, D.T.; Kettela, E.G.; Lucarotti, C.J. Application of balsam fir sawfly nucleopolyhedrovirus against its natural host Neodiprion abietis (Hymenoptera Diprionidae). In Proceedings of the IUFRO Workshop on Forest Insect Population Dynamics and Host Influences, Kanazawa, Japan, 14-19 September 2003; pp. 86–89.
[17]  Dwyer, G. The roles of density, stage, and patchiness in the transmission of an insect virus. Ecology 1991, 72, 559–574, doi:10.2307/2937196.
[18]  Dwyer, G.; Elkinton, J.L.; Buonaccorsi, J.P. Host heterogeneity in susceptibility and disease dynamics: tests of a mathematical model. Amer. Natur. 1997, 150, 685–707.
[19]  Moreau, G.; Eveleigh, E.S.; Lucarotti, C.J.; Quiring, D.T. Stage-specific responses to ecosystem alteration in an eruptive herbivorous insect. J. Appl. Ecol. 2006, 43, 28–34.
[20]  Anstey, L.J.; Quiring, D.T.; Ostaff, D.P. Seasonal changes in intra-tree distribution of immature balsam fir sawfly (Hymenoptera: Diprionidae). Can. Entomol. 2002, 134, 529–538, doi:10.4039/Ent134529-4.
[21]  Cory, J.S.; Hails, R.S.; Sait, S.M. Baculovirus ecology. In The Baculoviruses; Miller, L.K., Ed.; Plenum Publishing Corporation: New York, NY, USA, 1997; pp. 301–339.
[22]  Kaupp, W.J. Estimation of nuclear polyhedrosis virus produced in field populations of the European pine sawfly, Neodiprion sertifer (Geoff.) (Hymenoptera: Diprionidae). Can. J. Zool. 1982, 61, 1857–1861, doi:10.1139/z83-239.
[23]  Kaupp, W.J. Persistence of Neodiprion sertifer (Hymenoptera: Diprionidae) nuclear polyhedrosis virus on Pinus contortata foliage. Can. Entomol. 1983, 115, 869–873, doi:10.4039/Ent115869-7.
[24]  Olofsson, E. Environmental persistence of the nuclear polyhedrovirus of the European spruce sawfly in relation to epizootics in Swedish scots pine forests. J. Invertebr. Pathol. 1987, 52, 119–129, doi:10.1016/0022-2011(88)90110-3.
[25]  Olofsson, E. Persitence and dispersal of the nuclear polyhedrovirus of the European spruce sawfly (Geoffroy) [Hymenoptera:Diprionidae] in a virus-free lodgepole pine plantation in Sweden. Can. Entomol. 1988, 120, 887–892, doi:10.4039/Ent120887-10.
[26]  Olofsson, E. Dispersal of the nuclear polyhedrosis virus of Neodiprion sertifer from soil to pine foliage with dust. Entomol. Exper. Appl. 1988, 46, 181–186, doi:10.1111/j.1570-7458.1988.tb01109.x.
[27]  Olofsson, E. Transmission of the nuclear polyhedrosis virus of the european pine sawfly from adult to offspring. J. Invertebr. Pathol. 1989, 54, 322–330, doi:10.1016/0022-2011(89)90116-X.
[28]  Young, S.Y.; Yearian, W.C. Intra- and inter-colony transmission of a nuclear polyhedrosis virus of loblolly pine sawfly Neodiprion taedae linearis. J. Entomol. Sci. 1987, 22, 29–34.
[29]  Young, S.Y.; Yearian, W.C. Secondary transmission of a nuclear polyhedrosis virus of Neodiprion taedae [Hym.:Diprionidae] between larval colonies on loblolly pine. Entomophaga 1987, 34, 341–349.
[30]  Young, S.Y.; Yearian, W.C. The influence of canopy location on intercolony transmission of nuclear polyhedrosis virus in larval populations of loblolly pine sawfly Neodiprion taedae linearis. J. Entomol. Sci. 1990, 25, 535–537.
[31]  Bird, F.T. Transmission of some insect viruses with particular reference to ovarian transmission and its importance in the development of epizootics. J. Insect Pathol. 1961, 3, 352–380.
[32]  Dwyer, G.; Elkinton, J.S. Host dispersal and the spatial spread of insect pathogens. Ecology 1995, 76, 1262–1275, doi:10.2307/1940933.
[33]  D’Amico, V.; Elkinton, J.S.; Dwyer, G.; Burand, J.P.; Buonaccorsi, J.P. Virus transmission in gypsy moths is not a simple mass action process. Ecology 1996, 77, 201–206, doi:10.2307/2265669.
[34]  Vasconcelos, S.D.; Cory, J.S.; Wilson, K.R.; Sait, S.M.; Hails, R.S. Modified behaviour in baculovirus-infected lepidopteran larvae and its impact on the spatial distribution of inoculum. Biol. Control 1996, 7, 299–306, doi:10.1006/bcon.1996.0098.
[35]  Knell, R.J.; Begon, M.; Thompson, D.J. Transmission of Plodia interpunctella granulosis virus does not conform to the mass action model. J. Animal Ecol. 1998, 67, 592–599.
[36]  Beisner, E.B.; Meiers, J.H. Population density and transmission of virus in experimental populations of the western tent caterpillar (Lepidoptera: Lasiocamidae). Environ. Entomol. 1999, 28, 1107–1113.
[37]  Dwyer, G.; Dushoff, J.; Elkinton, J.; Levin, S. Pathogen-driven outbreaks in forest defoliators revisited: Building models from experimental data. Amer. Natur. 2000, 156, 105–120, doi:10.1086/303379.
[38]  Hails, R.S.; Hernandez-Crespo, P.; Sait, S.M.; Donnelly, C.A.; Green, B.M.; Cory, J.S. Transmission patterns of natural and recombinant baculoviruses. Ecology 2002, 83, 906–916.
[39]  D’Amico, V.; Elkinton, J.S.; Podgwaite, J.D.; Buonaccorsi, J.P.; Dwyer, G. Pathogen clumping: An explanation for non-linear transmission of an insect virus. Ecol. Entomol. 2005, 30, 383–390, doi:10.1111/j.0307-6946.2005.00697.x.
[40]  Anderson, R.M.; May, R.M. Population biology of infectious diseases: Part I. Nature 1979, 280, 361–367, doi:10.1038/280361a0.
[41]  Anderson, R.M.; May, R.M. Infectious diseases and population cycles of forest insects. Science 1980, 210, 658–661.
[42]  Anderson, R.M.; May, R.M. The population dynamics of microparasites and their invertebrate hosts. Phil. Trans. Roy. Soc. London B Biol. Sci. 1981, 291, 451–524, doi:10.1098/rstb.1981.0005.
[43]  Parsons, K.H.; Piene, H.; Farrell, J.; Quiring, D.T. Temporal patterns of balsam fir sawfly defoliation and growth loss in young balsam fir. Forest Ecol. Manag. 2003, 184, 33–46, doi:10.1016/S0378-1127(03)00145-2.
[44]  Parsons, K.; Quiring, D.; Piene, H.; Moreau, G. Relationship between balsam fir sawfly density and defoliation in balsam fir. Forest Ecol. Manag. 2005, 205, 325–331, doi:10.1016/j.foreco.2004.10.033.
[45]  Health Canada. Registration Decision Neodiprion Abietis Newfoundland Strain; Health Canada Pest Management Regulatory Agency: Ottawa, Canada, 2009; pp. 1–9.
[46]  Duffy, S.P.; Becker, E.M.; Whittome, B.; Lucarotti, C.J.; Levin, D.B. In vivo replication kinetics and transcription patterns of the balsam fir sawfly, Neodiprion abietis, nucleopolyhedrovirus. J. Gen. Virol. 2007, 88, 1945–1951, doi:10.1099/vir.0.82763-0.

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