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Screen in Saccharomyces cerevisiae for transposon insertion sites able to rescue phenotype of MTM1 deletion mutant using mTn-lacZ/LEU2 transposon library

Keywords: Keywords: Saccharomyces cerevisiae,mTn-lacZ/LEU2 transposon library,MTM1

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Objective] MTM1 gene is essential for superoxide dismutase 2 activity and normal mitochondrial functions. MTM1 deletion results in decreased superoxide dismutase 2 activity,impaired mitochondrial functions and growth defect on nonfermentable carbon source. To promote understanding of MTM1 gene,we started a genetic screen for transposon insertions which are able to rescue the growth defect resulting from MTM1 deletion. Methods] Routine screening strategy didn't work because of the irreversible damage cause...


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