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Cloning and Prokaryotic Expression of Viral Binding Protein(VBP) Gene From Endosybiotic Bacterium of Rhopalosiphum padi
禾谷缢管蚜体内的病毒结合蛋白基因的克隆与原核表达

Keywords: Myzus persicae,Endosymbiotic bacterium,Virus binding protein,Transmission
禾谷缢管蚜
,内共生细菌,病毒结合蛋白基因,传播

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Abstract:

Viral binding protein gene from Rhopalosiphum padi Yangling biotype was amplified by PCR method and then cloned.The complete nucleotide sequence of the gene was determined,It has 1647 nucleotides encoding 548 amino acids.Comparison showed this gene has 97% identity on nucleotide level with Buchnera groEL AM gene of Rhopalosiphum padi American biotype,while has 97.4% identity on amino acid level was found between this two genes.The VBP gene was ligated into pBV221 and pET30a expression vector and expressed the aim protein 63kD and 69kD.

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