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Cloning, expression and functional analysis of the genes in TPS/TPP trehalose synthetic pathway of Meiothermus ruber
红色亚栖热菌TPS/TPP海藻糖合成途径中相关基因的克隆、表达及功能鉴定

Keywords: Meiothermus ruber,trehalose,trehalose synthetic pathway,trehalose 6-phosphate synthase (TPS),trehalose 6-phosphate phosphatase (TPP)
红色亚栖热菌
,海藻糖,海藻糖合成途径,磷酸海藻糖合成酶(TPS),磷酸海藻糖磷酸酯酶(TPP)

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Abstract:

By constructing the genomic DNA library of Meiothermus ruber CBS-01, the genes of trehalose phosphate synthase (TPS) and trehalose phosphate phosphatase (TPP) involved in trehalose synthesis were cloned. The genes were cloned into the plasmid pET21a, and expressed in Escherichia coli Rosetta gami (DE3). The activities of these two purified enzymes were confirmed by thin layer chromatography (TLC). Meanwhile, we tested the cellular compatible solutes of M. ruber CBS-01 under different environmental pressure,...

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