Derma is progenitor cells sours, that are able to differentiate further in several mesodermal lineage and neural and endodermal lineage. Culture conditions, skin taking site and culture medium composition considerably contribute to it. Spheroid cultured mesenchymal dermal cells contribution to skin regeneration in granulating wound in rat model was estimated.
J. Toma, I. McKenzie, D. Bagli, et al., “Isolation and Characterization of Multipotent Skin-Derived Precursors from Human Skin,” Stem Cells, Vol. 23, No. 6, 2005, pp. 727-737. doi:10.1634/stemcells.2004-0134
J.-F. Lavoie, J. Biernaskie, Y. Chen, et al., “Skin-Derived Precursors Differentiate into Skeletogenic Cell Types and Contribute to Bone Repair,” Stem Cells and Development, Vol. 18, No. 6, 2009, pp. 893-905.
F. Chen, W. Zhang, D. Bi, et al., “Clonal Analysis of Nestin- Vimentin+ Multipotent Fibroblasts Isolated from Human Dermis,” Journal of Cell Science, Vol. 120, 2007, pp. 2875-2883. doi:10.1242/jcs.03478
I. Saburina and V. Repin, “3D-Culturing from Single Cells to the Regeneration of Tissue (the Question about the Phenomenon of Epithelial-Mesenchymal Plasticity),” Cell Transplantation and Tissue Engineering, Vol. 5, No. 2, 2010, pp. 75-86.
A. Gorkun, I. Saburina, N. Kosheleva, A. Pulin and V. Repin, “Induction of Vasculogenesis in the Spheroid of the Stromal Cells of Umbilical Cord,” Tula Journal New Medical Technologies, 2012, in press.