A Simple Cultural Method for Detection of Mycoplasma bovis

Simple method for the detection of Mycoplasma bovis in both liquid and solid cultures was established. Fresh media were easily prepared and dispatched into an eppendorf tube or a Petri dish, a new cultural procedure was followed with the eppendorf tube closed or the Petri dish sealed. Compared to the conventional methods, appropriate inoculating and subculturing operations got easier and a CO2 incubator was not required; moreover, probable contamination by airborne fungi or bacteria could be reduced or eliminated during long-term incubation. Similarly, M. bovis grew normally and could be investigated by Color-Change Units (CCU) enumeration, colony morphology or PCR assay.