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Isolation, Purification and Culture of Spermatogonia in Chicken

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To isolate, purify and culture spermotogonia from chicken testicular tissues, a procedure of enzymatic digestion and percoll density centrifugation was adopted for the single cell suspension to obtain purified spermatogonia. The results showed that, using the same purification method, the purity of spermatogonia gained from 6 days old chicken embryo was more than from 13, 15 and 19 days of age; adhesion purification step led to a harvest of 82% of total spermatogonia, which was 15.6% higher than that of direct isolation method; the adhesion time and survival time of spermotogonia before percoll density gradient centrifugation was earlier and longer than after precoll density gradient centrifugation.


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