Urinary Tract Infections (UTI) are among the most common bacterial infections in humans. The most frequent form of UTI is asymptomatic bacteriuria. The aim of this study is to determine the prevalence of asymptomatic bacteriuria on female students from Babol Islamic Azad University. This research has been carried out on 207 female students from Babol Islamic Azad University in 2002. The age ranges of the students were between 18-29 years. Mid Stream Urine (MSU) collected by students. Urinalysis and bacteriological identification culture of MSU is done on all of specimens and repeat two times in total. The prevalence of asymptomatic bacteriuria was determined by dividing the total number of cultures with evidence of asymptomatic bacteriuria by the total number of routine scheduled urine cultures performed. Bacteriological tests of MSU revealed that 19/207 (9/2%) were positive for asymptomatic bacteriuria. The microbe most frequently causes asymptomatic bacteriuria was Staphylococcus saprophyticus in 7/19 (36/8%) followed by Escherichia coli in 6/19 (31/6%) and Klebsiella in 3/19 (15/8%) each. asymptomatic bacteriuria are high rate in our study, therefore young women must obey the general rules of hygiene especially about genitourinary system to avoid increasing of asymptomatic bacteriuria rate.