Hepatitis is a general term meaning inflammation of the liver and can be caused by a variety of different viruses such as hepatitis A, B, C, D and E. Infection with HBV occurs worldwide and the prevalence rate is different in various areas. HBV causes one million deaths per annum. Hepatitis B vaccines have been used for reducing prevalence in many countries. The aim of this research is estimating efficacy of HBV Vaccine via HbsAb measurement in students who will be health-care workers in the future and presenting of non-responder persons. In this study, Blood samples of 288 students, who were vaccinated compactly, 1-6 month after third vaccination, were collected. After serum preparation, HbsAb were estimated by ELISA method. The antibody titer greater than 10 IU seems to be protective after the injection of Hepatitis B vaccine .In the HbsAb negative persons, vaccination was repeated. The results show the presence of HbsAb more than 10 IU in 250 individuals. Therefore the efficacy of vaccine in students is 86.8%. The average HbsAb production is 447.3 IU with 23.7 standard derivations. The lower limit was 0 and upper limit was 966 units. 25% of individuals have HbsAb less than 239, 25% of them 239-435, 25% of persons 435-653.7 and 25% of students more than 653-7 IU L 1. Also, in the second vaccination, 30 people had HbsAb more than 10 IU L 1. But 8 people (2.77%) were HbsAb negative or non-responder. The vaccine efficacy among students was same as that the other studies who reported 81-99%. In the mention of students who have HbsAb less than 10 IU L 1, Its better, storage conditions and the method of injecting vaccine and efficiency of available vaccine in Iran were studied. Also, study of serologic markers of HBV (HbcAb, HbeAg and HBeAb) for negative people is recommended.