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Quantitative Determination of Chlorpyrifos and Penconazole Residues in Grapes Using Gas Chromatography/Mass Spectrometry

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Abstract:

Samples of grape leaves and berries (CV: Zeini) were taken from grape-vine yards treated with chlorpyrifos (Dursban?) and penconazole (Ofir?) in order to determine their residues in these organs. The effect of time after spraying and number of sprays on the residues of both pesticides was studied. Gas chromatorgraphy /mass spectrometry (GC/MS) was used to determine the residue levels. Results obtained have indicated the presence of both pesticides in the tested leaf samples, but chlorpyrifos residues were detected in larger quantities than penconazole residues. Also, larger quantities of both pesticides were detected in the samples of berry cortex than in the samples of berry flesh. However, the residues of both pesticides in washing water of treated berries were lower than that in the cortex or in the flesh. This may indicate the systemic action of esticides especially penconazole, since washing the treated berries with water two weeks after application of the last spray did not remove the pesticides from berries. Overall results have indicated that the determined quantities of both pesticides residues especially in the cortical tissues of treated berries following the sixth application of both pesticides were higher than the quantities reported by other authors in grape berries, but they were generally less than the maximum residue limits (MRLS) defined by the residue legislations in other countries.

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